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Anti-Mouse anti DDDDK-Tag Monoclonal Antibody (CABE005)

SKU:
CABE005
Product Type:
Antibody
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
  • Protein Tags Anti-Mouse anti DDDDK-Tag Monoclonal Antibody CABE005
  • Protein Tags Anti-Mouse anti DDDDK-Tag Monoclonal Antibody CABE005
  • Protein Tags Anti-Mouse anti DDDDK-Tag Monoclonal Antibody CABE005
  • Protein Tags Anti-Mouse anti DDDDK-Tag Monoclonal Antibody CABE005
$419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Mouse anti DDDDK-Tag Monoclonal Antibody
Antibody SKU:CABE005
Antibody Size:100 uL
Application:WB IHC IF IP
Reactivity:
Host Species:Mouse
Immunogen:A synthetic peptide corresponding to DDDDK tag.
Application:WB IHC IF IP
Recommended Dilution:WB 1:2000 - 1:5000 IHC 1:50 - 1:500 IF 1:50 - 1:500 IP 1:50 - 1:100
Reactivity:
Positive Samples:293T
Immunogen:A synthetic peptide corresponding to DDDDK tag.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DYKD DDDK
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:Refer to Figures
Anti-Mouse anti DDDDK-Tag Monoclonal Antibody (CABE005)Western blot analysis of over-expressed DDDDK-tagged protein in 293T cells using Mouse anti DDDDK-Tag antibody (CABE005) at different dilution.Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-Mouse anti DDDDK-Tag Monoclonal Antibody (CABE005)Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (CABE005). Blue: DAPI for nuclear staining.
Anti-Mouse anti DDDDK-Tag Monoclonal Antibody (CABE005)Immunofluorescence analysis of HeLa cells transfected with DDDDK-Tag and untreated HeLa cells use Mouse anti DDDDK-Tag mAb(CABE005) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-Mouse anti DDDDK-Tag Monoclonal Antibody (CABE005)Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells using DDDDK-tag antibody (CABE005). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Synonyms:DDDDK, DDDDK tag, DDDDK-tag
Background:FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

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