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Anti-Mouse anti HA-Tag Monoclonal Antibody (CABE008)

SKU:
CABE008
Product Type:
Antibody
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
  • Protein Tags Anti-Mouse anti HA-Tag Monoclonal Antibody CABE008
  • Protein Tags Anti-Mouse anti HA-Tag Monoclonal Antibody CABE008
  • Protein Tags Anti-Mouse anti HA-Tag Monoclonal Antibody CABE008
$419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Mouse anti HA-Tag Monoclonal Antibody
Antibody SKU:CABE008
Antibody Size:100 uL
Application:WB IF IP
Reactivity:
Host Species:Mouse
Immunogen:A synthetic peptide corresponding to HA tag.
Application:WB IF IP
Recommended Dilution:WB 1:2000 - 1:5000 IF 1:50 - 1:500 IP 1:20 - 1:50
Reactivity:
Positive Samples:293T
Immunogen:A synthetic peptide corresponding to HA tag.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YPYD VPDY A
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:Refer to Figures
Anti-Mouse anti HA-Tag Monoclonal Antibody (CABE008)Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody (CABE008) at different dilution. Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Mouse anti HA-Tag Monoclonal Antibody (CABE008)Immunofluorescence analysis of 293T cells transfected with HA-Tag fusion protein and untreated 293T cells use Mouse anti HA-Tag mAb (CABE008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Anti-Mouse anti HA-Tag Monoclonal Antibody (CABE008)Immunoprecipitation of over-expressed HA-tagged protein in 293T cells using HA-tag antibody (CABE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Synonyms:HA, HA tag, HA-tag
Background:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

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