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Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)

SKU:
CABE003
Product Type:
Antibody
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
  • Protein Tags Anti-Mouse anti His-Tag Monoclonal Antibody CABE003
  • Protein Tags Anti-Mouse anti His-Tag Monoclonal Antibody CABE003
  • Protein Tags Anti-Mouse anti His-Tag Monoclonal Antibody CABE003
  • Protein Tags Anti-Mouse anti His-Tag Monoclonal Antibody CABE003
$419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Mouse anti His-Tag Monoclonal Antibody
Antibody SKU:CABE003
Antibody Size:100 uL
Application:WB IF IP
Reactivity:
Host Species:Mouse
Immunogen:A synthetic peptide corresponding to His tag.
Application:WB IF IP
Recommended Dilution:WB 1:2000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:
Positive Samples:293T
Immunogen:A synthetic peptide corresponding to His tag.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HHHH HH
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:Refer to Figures
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein, using His-Tag antibody (CABE003) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein, using His-Tag antibody (CABE003) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (CABE003) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (CABE003). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Synonyms:His, His tag, His-tag
Background:Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

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