The Assay Genie Mouse Flt3L (FMS-like Tyrosine Kinase 3 Ligand) ELISA Kit is meticulously crafted to allow the precise quantification of Flt3L levels in various biological samples derived from mouse models. Flt3L, a critical growth factor pivotal in hematopoiesis and immune cell development, plays a vital role in regulating the differentiation, proliferation, and survival of hematopoietic stem cells and dendritic cells. Accurate measurement of Flt3L is crucial for understanding its role in immune modulation, hematopoiesis, and disease pathogenesis. Our Flt3L ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results for your research investigations. Manufactured under stringent quality control processes, this kit delivers consistent performance and user-friendly protocols that facilitate ease of use, making it an excellent research tool for studying immune responses, hematopoiesis, and related biological processes. Trust Assay Genie's Flt3L ELISA Kit for precise and dependable quantification of FMS-like Tyrosine Kinase 3 Ligand in your mouse model studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
