MTS Cell Proliferation Assay Kit (BN00551)

SKU:: BN00551
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€385 - €1,742

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Description

MTS Cell Proliferation Assay Kit

Assay Genie's MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 490-500 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.

Assay Genie's MTS assay is performed by adding the reagent directly into the cell culture media without the intermittent steps, which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in 96-well microtiter plate.

Figure: Effect of cell number on absorbance at 490 nm. Various numbers of Jurkat cells were cultured in a final volume of 200 µl/well by incubating overnight at 37°C. MTS reagent was added and absorbance at 490 nm was recorded using ELISA plate reader. Each point represents a mean of 3 replicates. Assay was performed according to the kit protocol.

Key Information	Description
Kit Summary	Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals Assessment of physiological mediators that inhibit cell growth
Size	250 Assays 500 Assays 2500 Assays 5000 Assays 10,000 Assays
Detection Methods	Absorbance (490-500 nm)
Applications	Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators that inhibit cell growth.
Features and Benefits	Simple one-step procedure; takes ~ 4 hours High-throughput. The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.
Kit Contents	MTS Reagent (in electrocoupling solution)
Storage	-20°C
Shipping Conditions	Gel Pack
Usage	For Research Use Only! Not For Use in Humans.