MTS Cell Proliferation Assay Kit (BN00551)
- SKU:
- BN00551
Description
MTS Cell Proliferation Assay Kit
Assay Genie's MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 490-500 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.
Assay Genie's MTS assay is performed by adding the reagent directly into the cell culture media without the intermittent steps, which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in 96-well microtiter plate.
Figure: Effect of cell number on absorbance at 490 nm. Various numbers of Jurkat cells were cultured in a final volume of 200 µl/well by incubating overnight at 37°C. MTS reagent was added and absorbance at 490 nm was recorded using ELISA plate reader. Each point represents a mean of 3 replicates. Assay was performed according to the kit protocol.
| Key Information | Description |
| Kit Summary |
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| Size |
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| Detection Methods | Absorbance (490-500 nm) |
| Applications |
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| Features and Benefits |
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| Kit Contents | MTS Reagent (in electrocoupling solution) |
| Storage | -20°C |
| Shipping Conditions | Gel Pack |
| Usage | For Research Use Only! Not For Use in Humans. |
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