Myeloperoxidase (MPO) Colorimetric Activity Assay Kit (BN00959)

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BN00959
  • Myeloperoxidase (MPO) Colorimetric Activity Assay Kit (BN00959)
  • Myeloperoxidase (MPO) Colorimetric Activity Assay Kit (BN00959)
  • Myeloperoxidase (MPO) Colorimetric Activity Assay Kit (BN00959)
€667

Description

ELISA Kit Technical ManualMSDS

Myeloperoxidase (MPO) Colorimetric Activity Assay Kit

Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. MPO contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H and chloride anion (Cl-, or the equivalent from a non-chlorine halide). MPO also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In Assay Genie's MPO Assay Kit, the HClO produced from Hand Cl- is reacted with taurine to generate the taurine chloramine, which subsequently reacts with the TNB2- probe to eliminate color (= 412 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of MPO. This kit can be used to detect MPO as low as 0.05 mU per well.

Figure: (a) TNB Standard Curve. (b) Measurement of MPO activity using WBC lysate (3 µg), and MPO Positive Control (5 µl) and (10 µl). (c) MPO specific activity in WBC lysate. Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00959

Size

100 assays

Detection Method

Absorbance (412 nm)

Species Reactivity

Mammalian

Applications

N/A

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assays for measuring MPO as low as 0.05 mU per well

Kit Components

  • MPO Assay Buffer
  • DTNB Probe
  • TCEP
  • MPO Substrate
  • Stop mix
  • MPO Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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