MPO Rabbit Polyclonal Antibody (CAB1374)

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SKU:
CAB1374
  • Western blot analysis of extracts of HL-60 cells, using MPO antibody (CAB1374) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat spleen using MPO Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using MPO Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of Mouse bone marrow cells using MPO antibody (CAB1374) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human colon carcinoma cells using MPO antibody (CAB1374) at dilution of 1:100. Blue: DAPI for nuclear staining.
€99 - €249

Description

Technical Manual

MPO Rabbit Polyclonal Antibody

Product Name:MPO Rabbit Polyclonal Antibody
Product SKU:CAB1374
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-310 of human MPO (NP_000241.1).
Sequence:

APAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYFKQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAYQDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVKRNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFVTGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCI

Gene ID:4353
Swiss Prot:P05164
Synonyms:MPO
Calculated MW:73kDa/83kDa/87kDa
Observed MW:60KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:HL-60
Cellular Location:Lysosome

Product Images

MPO Rabbit Polyclonal Antibody (CAB1374)

Western blot analysis of extracts of HL-60 cells, using MPO antibody (CAB1374) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

MPO Rabbit Polyclonal Antibody (CAB1374)

Immunohistochemistry of paraffin-embedded rat spleen using MPO Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens).

MPO Rabbit Polyclonal Antibody (CAB1374)

Immunohistochemistry of paraffin-embedded mouse spleen using MPO Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens).

MPO Rabbit Polyclonal Antibody (CAB1374)

Immunofluorescence analysis of Mouse bone marrow cells using MPO antibody (CAB1374) at dilution of 1:100. Blue: DAPI for nuclear staining.

MPO Rabbit Polyclonal Antibody (CAB1374)

Immunofluorescence analysis of Human colon carcinoma cells using MPO antibody (CAB1374) at dilution of 1:100. Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function:heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; defense response; response to yeast; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease

NCBI Summary:Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
UniProt Code:P05164
NCBI GenInfo Identifier:129825
NCBI Gene ID:4353
NCBI Accession:P05164.1
UniProt Secondary Accession:P05164,Q14862, Q4PJH5, Q9UCL7, A1L4B8,
UniProt Related Accession:P05164
Molecular Weight:87,248 Da
NCBI Full Name:Myeloperoxidase
NCBI Synonym Full Names:myeloperoxidase
NCBI Official Symbol:MPO 
NCBI Protein Information:myeloperoxidase
UniProt Protein Name:Myeloperoxidase
Protein Family:Myeloperoxidase
UniProt Gene Name:MPO 
UniProt Entry Name:PERM_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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