Anti-MYH2 Antibody (CAB15292)

Antibody Name:	Anti-MYH2 Antibody
Antibody SKU:	CAB15292
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle, Rat skeletal muscle

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SFKN KLYD QHLG KSAN FQKP KVVK GKAE AHFA LIHY AGVV DYNI TGWL EKNK DPLN ETVV GLYQ KSAM KTLA QLFS GAQT AEGE GAGG GAKK GGKK KGSS F
Gene ID:	4620
Uniprot:	Q9UKX2
Cellular Location:	Cytoplasm, myofibril
Calculated MW:	79kDa/223kDa
Observed MW:	223kDa

Synonyms:

MYH2, IBM3, MYH2A, MYHSA2, MYHas8, MYPOP, MyHC-2A, MyHC-IIa, myosin-2

Background:

Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	MYH2: Muscle contraction. Required for cytoskeleton organization. Defects in MYH2 are the cause of inclusion body myopathy type 3 (IBM3). Hereditary inclusion body myopathies constitute a group of neuromuscular disorders characterized by slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM3 is a variant of hereditary inclusion body myopathies and is characterized by autosomal dominant myopathy with joint contracture, ophthalmoplegia and rimmed vacuoles. Morphological analysis of muscle biopsies from patients indicate that the type 2A fibers frequently were abnormal, whereas other fiber types appeared normal.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: Golgi apparatus; sarcomere; protein complex; myofibril; contractile ring; muscle myosin complex; intercellular junction; cytosol; A band Molecular Function:microfilament motor activity; calmodulin binding; protein binding; actin binding; ATP binding Biological Process: muscle contraction; plasma membrane repair; metabolic process; innate immune response; response to activity; muscle filament sliding Disease: Inclusion Body Myopathy 3, Autosomal Dominant
NCBI Summary:	Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
UniProt Code:	Q9UKX2
NCBI GenInfo Identifier:	13431716
NCBI Gene ID:	4620
NCBI Accession:	Q9UKX2.1
UniProt Secondary Accession:	Q9UKX2,Q14322, Q16229, Q567P6, Q86T56, A0AVL4,
UniProt Related Accession:	Q9UKX2
Molecular Weight:	1941
NCBI Full Name:	Myosin-2
NCBI Synonym Full Names:	myosin, heavy chain 2, skeletal muscle, adult
NCBI Official Symbol:	MYH2
NCBI Official Synonym Symbols:	IBM3; MYH2A; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
NCBI Protein Information:	myosin-2; myosin heavy chain 2; myosin heavy chain 2a; myosin heavy chain IIa; fast 2a myosin heavy chain; type IIA myosin heavy chain; myosin heavy chain, skeletal muscle, adult 2; inclusion body myopathy 3, autosomal dominant; myosin, heavy polypeptide 2, skeletal muscle, adult
UniProt Protein Name:	Myosin-2
UniProt Synonym Protein Names:	Myosin heavy chain 2; Myosin heavy chain 2a; MyHC-2a; Myosin heavy chain IIa; MyHC-IIa; Myosin heavy chain, skeletal muscle, adult 2
UniProt Gene Name:	MYH2
UniProt Entry Name:	MYH2_HUMAN

	Western blot analysis of extracts of various cell lines, using MYH2 antibody (CAB15292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunofluorescence analysis of mouse skeletal muscle using MYH2 Polyclonal Antibody (CAB15292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
MYH2 Antibody (PACO10682)	Human MYH2 (Myosin-2) ELISA Kit
	Human Myosin-2 (MYH2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)