NAD+/NADH Colorimetric Assay Kit (384-well) (BN01118)

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BN01118
  • NAD+/NADH Colorimetric Assay Kit (384-well) (BN01118)
  • NAD+/NADH Colorimetric Assay Kit (384-well) (BN01118)
  • NAD+/NADH Colorimetric Assay Kit (384-well) (BN01118)
€738

Description

ELISA Kit Technical ManualMSDS

NAD+/NADH Colorimetric Assay Kit (384-well)

Nicotinamide Adenine Dinucleotide (NAD) is a co-enzyme indispensible for the activity of multiple catalytic proteins. NAD+ and NADH, its reduced form, participate in many biological redox reactions. NAD+ is mostly associated with redox enzymes required for catabolic reaction and energy production. Assay Genie's NADH/NAD Quantification Kit provides a convenient tool for a sensitive detection of NADH, NAD and their ratio. There is no requirement to purify NADH or NAD from samples. The NADH selective developer develops color while reacting with NADH and can be detected at OD 450 nm. The total NAD can be easily quantified by comparing with the provided NADH standard. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit can detect as low as 0.04 pmol of NADH in a 384 well assay plate. The reaction specifically detects NADH and NAD, but not NADP nor NADPH.

Figure: a) Standard Curve for NADH, NAD and heated NADH (Ht-NADH) and NAD (Ht-NAD); both heated 30 min at 60 °C; (b) Quantitation of NADH in freshly processed rat liver and kidney tissue. Tissue homogenates were prepared following kit’s protocol and deproteinized by 10 kDa spin column (12000 x g, 10 min, 4°C). 5 µl filtrates were assayed according to the kit’s protocol. (c) Quantitation of NADH in human serum and urine. Samples were centrifuged and supernatant was collected and deproteinized by 10 kDa spin column. Filtrate was spiked with 10 pmol of NADH Standard, assayed following kit’s protocol. All standards and samples absorbance readings were taken after 60 minutes cycling reaction at room temperature.

Key Information Description

Product SKU

BN01118

Size

400 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

Assay Genie's NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio

Features and Benefits

  • Simple procedure; takes ~2 hours
  • Fast and convenient
  • Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.

Kit Components

  • NADH/NAD Extraction Buffer
  • NAD Cycling Buffer
  • NAD Cycling Enzyme Mix
  • NADH Developer
  • Stop Solution
  • NADH Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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