Neuraminidase Activity Fluorometric Assay Kit (BN00951)

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BN00951
  • Neuraminidase Activity Fluorometric Assay Kit (BN00951)
  • Neuraminidase Activity Fluorometric Assay Kit (BN00951)
  • Neuraminidase Activity Fluorometric Assay Kit (BN00951)
€601

Description

ELISA Kit Technical ManualMSDS

Neuraminidase Activity Fluorometric Assay Kit

Neuraminidase (NA) is a very common enzyme that hydrolyzes terminal sialic acid residues on polysaccharide chains; most often a galactose residue. NA activity plays a key role in the invasion of target cells and the replication of influenza virus. NA activity also assists in the elution of progeny viruses from infected cells, and prevents self-aggregation of virus. Thus, NA is an important target for drug development. Assay Genie's Neuraminidase Activity Assay Kit provides a simple and sensitive method for measuring NA activity using fluorescence (Ex/Em = 530/590 nm). The assay utilizes NA Probe to detect the neuraminidase activity. This high-throughput adaptable assay kit can detect NA activity as low as 2.0 mU/ml in a variety of samples.

Figure: (a) Galactose Standard Curve. (b) NA activity in normal human serum (1 µl) Positive Control (1 µl). (c) Calculated activity of serum. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00951

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 530/590 nm)

Applications

Measurement of NA activity in various tissues/cells extracts or serum sample infected with influenza virus.

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect Neuraminidase activity < 2 mU/ml in a variety of samples.

Kit Components

  • NA Assay Buffer
  • NA Probe (in DMSO, anhydrous)
  • NA Substrate (Lyophilized)
  • NA Enzyme Mix I (Lyophilized)
  • NA Enzyme Mix II (Lyophilized)
  • Galactose Standard (100 nmol/µl)
  • NA Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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