Anti-NOS2 Antibody (PACO61566)

Antibody Name:	NOS2 Antibody (PACO61566)
Antibody SKU:	PACO61566
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Nitric oxide synthase, inducible protein (17-141AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Immunofluorescence staining of A549 cells with PACO61566 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.

Synonyms:

Nitric oxide synthase, inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, NOS2A

UniProt Protein Function:	Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
UniProt Protein Details:	Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group By similarity.Binds 1 FAD By similarity.Binds 1 FMN By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. Enzyme regulation: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity By similarity. Subunit structure: Homodimer. Binds SLC9A3R1. Tissue specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Induction: By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Ref.14 Polymorphism: Note=Genetic variations in NOS2 are involved in resistance to malaria [ MIM:611162]. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.
NCBI Summary:	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
UniProt Code:	P35228
NCBI GenInfo Identifier:	1352513
NCBI Gene ID:	4843
NCBI Accession:	P35228.2
UniProt Secondary Accession:	P35228,O60757, O94994, Q16263, Q16692, Q4TTS5, Q9UD42 A1L3U5, B7ZLY2,
UniProt Related Accession:	P35228
Molecular Weight:	131,117 Da
NCBI Full Name:	Nitric oxide synthase, inducible
NCBI Synonym Full Names:	nitric oxide synthase 2, inducible
NCBI Official Symbol:	NOS2
NCBI Official Synonym Symbols:	NOS; INOS; NOS2A; HEP-NOS
NCBI Protein Information:	nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
UniProt Protein Name:	Nitric oxide synthase, inducible
UniProt Synonym Protein Names:	Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Protein Family:	Inositol 3-kinase
UniProt Gene Name:	NOS2
UniProt Entry Name:	NOS2_HUMAN

Antibodies	ELISA Kits
Anti-iNOS Antibody (CAB0312)	Human iNOS / Nitric oxide synthase, inducible ELISA Kit
Anti-NOS2 Antibody (CAB14031)	Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit (HUES00472)
Anti-iNOS Antibody (CAB3200)	Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit (HUES01897)
Anti-NOS2 Antibody (CAB18247)	Human Nitric oxide synthase, inducible (NOS2) ELISA Kit
Phospho-NOS2 (Y151) Antibody (PACO04450)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)