Anti-Niemann Pick C1 Antibody (CAB4795)

Antibody Name:	Anti-Niemann Pick C1 Antibody (CAB4795)
Antibody SKU:	CAB4795
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Niemann Pick C1.

Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse liver, Rat lung

Immunogen:	A synthesized peptide derived from human Niemann Pick C1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:	Late endosome membrane, Lysosome membrane, Multi-pass membrane protein
Calculated MW:	180kDa
Observed MW:	180KDa

Synonyms:

NPC1, NPC, Niemann-Pick C1 protein

Background:

This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.

	Immunofluorescence analysis of HepG2 cells using Niemann Pick C1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using Niemann Pick C1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
	Immunohistochemistry of paraffin-embedded rat ovary using Niemann Pick C1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse liver using Niemann Pick C1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse kidney using Niemann Pick C1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using Niemann Pick C1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Western blot analysis of extracts of Mouse liver, using Niemann Pick C1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.