SARS-CoV-2 Nucleocapsid Monoclonal Antibody [1A6] HRP-conjugated

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SKU:
PACOV0009
€389

Description

SARS-CoV-2 Nucleocapsid Monoclonal Antibody [1A6] HRP-conjugated

Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.

Antibody Size: 100ul

Function

Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.

Information Description

Uniprot No.

P0DTC9

Target Names

N

Alternative Names

Nucleocapsid protein, NC, protein N, N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

HRP

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

IA6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Subcellular Location

Virion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus

Protein Families

Betacoronavirus nucleocapsid protein family

SARS-CoV-2 Nucleocapsid Information

Key Information Description

Uniprot:

Protein:

Nucleoprotein

Gene:

N

Organism:

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Alternative Names:

Nucleocapsid protein, Nucleoprotein, NC, Protein N

Nucleocapsid function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).

Sub-cellular location

    Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.

Post-Translational Modification

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.

Phosphorylated on serine and threonine residues (By similarity). Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis.

Interaction

Homooligomer. Both monomeric and oligomeric forms interact with RNA.

Interacts with protein M (PubMed:15351485).

Interacts with protein E (PubMed:24766657). May bind to host HNRNPA1 (Probable).

Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (By similarity). May interact with host SMAD3 (Probable).

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