Total Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric) (BN00068)

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BN00068
  • Total Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric) (BN00068)
  • Total Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric) (BN00068)
  • Total Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric) (BN00068)
  • Total Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric) (BN00068)
€1,038

Description

ELISA Kit Technical ManualMSDS

Total Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. ExoGenie is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. ExoGenie consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

Figure: CD9 Titration of COLO1 (1) and plasma (2) exosomes. Example of standard curves obtained. Exosome standard preparation derived from (1) COLO1 Cell Culture Supernatant and (2) Plasma of healthy donors. This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay. (3) CD9 titration of plasma healthy donor exosome standards. (4) CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.

Key Information Description

Product SKU

BN00068

Size

100 assays

Detection Method

Colorimetric Assay

Species Reactivity

Cell media

Applications

Overall exosome immunocapture and quantification from cell culture supernatant

Features and Benefits

Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes

Kit Components

  • Sample buffer (1X)
  • Washing buffer (25X)
  • Primary Antibody
  • HRP conjugated
  • Substrate chromogenic solutions
  • Stop solution
  • Exosome Standards
  • Immunoplate

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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