Anti-P2RX7 Antibody (CAB10511)

Antibody Name:	Anti-P2RX7 Antibody
Antibody SKU:	CAB10511
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 47-334 of human P2RX7 (NP_002553.3).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A375, HT-29, U-251MG, LO2, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 47-334 of human P2RX7 (NP_002553.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SDKL YQRK EPVI SSVH TKVK GIAE VKEE IVEN GVKK LVHS VFDT ADYT FPLQ GNSF FVMT NFLK TEGQ EQRL CPEY PTRR TLCS SDRG CKKG WMDP QSKG IQTG RCVV YEGN QKTC EVSA WCPI EAVE EAPR PALL NSAE NFTV LIKN NIDF PGHN YTTR NILP GLNI TCTF HKTQ NPQC PIFR LGDI FRET GDNF SDVA IQGG IMGI EIYW DCNL DRWF HHCR PKYS FRRL DDKT TNVS LYPG YNFR YAKY YKEN NVEK RTLI KVFG IRFD ILVF GTGG KFDI IQLV
Gene ID:	5027
Uniprot:	Q99572
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	14kDa/31kDa/35kDa/41kDa/49kDa/58kDa/68kDa
Observed MW:	65kDa

Synonyms:

P2RX7, P2X7

Background:

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.

UniProt Protein Function:	P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.
UniProt Protein Details:	Protein type:Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc. Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytoplasm; integral to nuclear inner membrane; integral to plasma membrane; membrane; plasma membrane Molecular Function:ATP binding; ATP-gated cation channel activity; lipopolysaccharide binding; protein binding; purinergic nucleotide receptor activity; receptor binding Biological Process: bleb formation; blood coagulation; cell surface receptor linked signal transduction; membrane depolarization; negative regulation of bone resorption; negative regulation of cell volume; negative regulation of MAPKKK cascade; phospholipid scrambling; pore complex biogenesis; positive regulation of bone mineralization; positive regulation of cytolysis; positive regulation of cytoskeleton organization and biogenesis; positive regulation of glycolysis; positive regulation of interleukin-1 beta secretion; regulation of sodium ion transport; response to ATP; sensory perception of pain Disease: Leukemia, Chronic Lymphocytic
NCBI Summary:	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]
UniProt Code:	Q99572
NCBI GenInfo Identifier:	395398617
NCBI Gene ID:	5027
NCBI Accession:	Q99572.4
UniProt Secondary Accession:	Q99572,O14991, Q4VKH8, Q4VKH9, Q4VKI0, Q4VKI1, Q4VKI2 A8K2Z0, E7EMK6, F5H6P2, F5H7E8, F8W951,
UniProt Related Accession:	Q99572
Molecular Weight:	69kDa
NCBI Full Name:	P2X purinoceptor 7
NCBI Synonym Full Names:	purinergic receptor P2X 7
NCBI Official Symbol:	P2RX7
NCBI Official Synonym Symbols:	P2X7
NCBI Protein Information:	P2X purinoceptor 7
UniProt Protein Name:	P2X purinoceptor 7
UniProt Synonym Protein Names:	ATP receptor; P2Z receptor; Purinergic receptor
Protein Family:	P2X purinoceptor
UniProt Gene Name:	P2RX7

	Western blot analysis of extracts of various cell lines, using P2RX7 antibody (CAB10511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using P2RX7 antibody (CAB10511) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
P2RX7 Antibody (PACO11039)	Human P2RX7 / P2X purinoceptor 7 ELISA Kit
	Human P2X purinoceptor 7 (P2RX7) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)