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PARP1 Polyclonal Antibody (CAB21365)

SKU:
CAB21365
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
IF
€139 - €529
Frequently bought together:

Description

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Product Name:PARP1 Polyclonal Antibody
SKU:CAB21365
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PARP1 (NP_001609.2).
Sequence:PGVK SEGK RKGD EVDG VDEV AKKK SKKE KDKD SKLE KALK AQND LIWN IKDE LKKV CSTN DLKE LLIF NKQQ VPSG ESAI LDRV ADGM VFGA LLPC EECS G
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:2000 - 1:6000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; P1
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Nucleus, nucleolus.
Calculated MW:113kDa
Observed MW:114kDa

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Purification Method:Affinity purification
Gene ID:142
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type(WT) and PARP1 knockout (KO) HeLa cells, using [KO Validated] PARP1 Rabbit pAb (CAB21365) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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