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Anti-PC Antibody (CAB6301)

SKU:
CAB6301
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-PC Antibody CAB6301
  • Metabolism Antibodies 2 Anti-PC Antibody CAB6301
  • Metabolism Antibodies 2 Anti-PC Antibody CAB6301
€139 - €419
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Description

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Antibody Name:Anti-PC Antibody
Antibody SKU:CAB6301
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human PC (NP_001035806.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HeLa, MCF7, A-549, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human PC (NP_001035806.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HSMG LGSK FKEV KKAY VEAN QMLG DLIK VTPS SKIV GDLA QFMV QNGL SRAE AEAQ AEEL SFPR SVVE FLQG YIGV PHGG FPEP FRSK VLKD LPRV EGRP GASL PPLD LQAL EKEL VDRH GEEV TPED VLSA AMYP DVFA HFKD FTAT FGPL DSLN TRLF LQGP KIAE EFEV ELER GKTL HIKA LAVS DLNR AGQR QVFF ELNG QLRS ILVK DTQA MKEM HFHP KALK DVKG QIGA PMPG KVID IKVV AGAK VAKG QPLC VLSA MKME TVVT SPME GTVR KVHV TKDM TLEG DDLI LEIE
Gene ID:5091
Uniprot:P11498
Cellular Location:Mitochondrion matrix
Calculated MW:57kDa/129kDa
Observed MW:129kDa
Synonyms:PC, PCB
Background:This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
UniProt Protein Function:PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
UniProt Protein Details:

Protein type:EC 6.4.1.1; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Ligase

Chromosomal Location of Human Ortholog: 11q13.4-q13.5

Cellular Component: cytoplasm; cytosol; mitochondrial matrix; mitochondrion

Molecular Function:protein binding; pyruvate carboxylase activity

Biological Process: biotin metabolic process; gluconeogenesis; viral RNA genome packaging

Disease: Pyruvate Carboxylase Deficiency

NCBI Summary:This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P11498
NCBI GenInfo Identifier:1709947
NCBI Gene ID:5091
NCBI Accession:P11498.2
UniProt Secondary Accession:P11498,Q16705, B4DN00,
UniProt Related Accession:P11498
Molecular Weight:57,925 Da
NCBI Full Name:Pyruvate carboxylase, mitochondrial
NCBI Synonym Full Names:pyruvate carboxylase
NCBI Official Symbol:PC  
NCBI Official Synonym Symbols:PCB  
NCBI Protein Information:pyruvate carboxylase, mitochondrial
UniProt Protein Name:Pyruvate carboxylase, mitochondrial
UniProt Synonym Protein Names:Pyruvic carboxylase; PCB
Protein Family:PCTP-like protein
UniProt Gene Name:PC  
UniProt Entry Name:PYC_HUMAN
Anti-PC Antibody (CAB6301)Western blot analysis of extracts of various cell lines, using PC antibody (CAB6301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-PC Antibody (CAB6301)Immunofluorescence analysis of U2OS cells using PC antibody (CAB6301). Blue: DAPI for nuclear staining.
Anti-PC Antibody (CAB6301)Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug PC antibody (CAB6301). Western blot was performed from the immunoprecipitate using PC antibody (CAB6301) at a dilition of 1:1000.

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