Anti-PCBP1 Antibody (CAB1044)

Antibody Name:	Anti-PCBP1 Antibody
Antibody SKU:	CAB1044
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PCBP1 (NP_006187.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3, HepG2, MCF7, Mouse liver, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PCBP1 (NP_006187.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LRLV VPAT QCGS LIGK GGCK IKEI REST GAQV QVAG DMLP NSTE RAIT IAGV PQSV TECV KQIC LVML ETLS QSPQ GRVM TIPY QPMP ASSP VICA GGQD R
Gene ID:	5093
Uniprot:	Q15365
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	37kDa
Observed MW:	37kDa

Synonyms:

PCBP1, HEL-S-85, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X

Background:

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

UniProt Protein Function:	hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
UniProt Protein Details:	Protein type:Translation; RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 2p13-p12 Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane; nucleoplasm Molecular Function:mRNA binding; protein binding; RNA binding; single-stranded DNA binding Biological Process: gene expression; nuclear mRNA splicing, via spliceosome
NCBI Summary:	This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15365
NCBI GenInfo Identifier:	42560548
NCBI Gene ID:	5093
NCBI Accession:	Q15365.2
UniProt Secondary Accession:	Q15365,Q13157, Q14975,
UniProt Related Accession:	Q15365
Molecular Weight:	37,498 Da
NCBI Full Name:	Poly(rC)-binding protein 1
NCBI Synonym Full Names:	poly(rC) binding protein 1
NCBI Official Symbol:	PCBP1
NCBI Official Synonym Symbols:	HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
NCBI Protein Information:	poly(rC)-binding protein 1
UniProt Protein Name:	Poly(rC)-binding protein 1
UniProt Synonym Protein Names:	Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3
Protein Family:	Poly(rC)-binding protein
UniProt Gene Name:	PCBP1
UniProt Entry Name:	PCBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using PCBP1 antibody (CAB1044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using PCBP1 antibody (CAB1044) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ELISA Kits

Human PCBP1 (Poly (rC)-binding protein 1) ELISA Kit (HUFI04950)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)