Anti-PDIA3 Antibody (CAB1085)

Antibody Name:	Anti-PDIA3 Antibody
Antibody SKU:	CAB1085
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-505 of human PDIA3 (NP_005304.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3, MCF7, Jurkat, HepG2, LO2, Mouse brain, Mouse thymus, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-505 of human PDIA3 (NP_005304.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FRPS HLTN KFED KTVA YTEQ KMTS GKIK KFIQ ENIF GICP HMTE DNKD LIQG KDLL IAYY DVDY EKNA KGSN YWRN RVMM VAKK FLDA GHKL NFAV ASRK TFSH ELSD FGLE STAG EIPV VAIR TAKG EKFV MQEE FSRD GKAL ERFL QDYF DGNL KRYL KSEP IPES NDGP VKVV VAEN FDEI VNNE NKDV LIEF YAPW CGHC KNLE PKYK ELGE KLSK DPNI VIAK MDAT ANDV PSPY EVRG FPTI YFSP ANKK LNPK KYEG GREL SDFI SYLQ REAT NPPV IQEE KPKK KKKA QEDL
Gene ID:	2923
Uniprot:	P30101
Cellular Location:	Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome
Calculated MW:	56kDa
Observed MW:	57kDa

Synonyms:

PDIA3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC

Background:

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates.

UniProt Protein Function:	GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.
UniProt Protein Details:	Protein type:Chaperone; Endoplasmic reticulum; Protease; Isomerase; EC 5.3.4.1; Apoptosis; Phospholipase Chromosomal Location of Human Ortholog: 15q15 Cellular Component: smooth endoplasmic reticulum; cell surface; focal adhesion; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; nucleus Molecular Function:disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER
NCBI Summary:	This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
UniProt Code:	P30101
NCBI GenInfo Identifier:	2507461
NCBI Gene ID:	2923
NCBI Accession:	P30101.4
UniProt Secondary Accession:	P30101,Q13453, Q14255, Q8IYF8, Q9UMU7,
UniProt Related Accession:	P30101,AAB37397
Molecular Weight:	56,782 Da
NCBI Full Name:	Protein disulfide-isomerase A3
NCBI Synonym Full Names:	protein disulfide isomerase family A, member 3
NCBI Official Symbol:	PDIA3
NCBI Official Synonym Symbols:	P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
NCBI Protein Information:	protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
UniProt Protein Name:	Protein disulfide-isomerase A3
UniProt Synonym Protein Names:	58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
Protein Family:	Protein disulfide-isomerase
UniProt Gene Name:	PDIA3
UniProt Entry Name:	PDIA3_HUMAN

	Western blot analysis of extracts of various cell lines, using PDIA3 antibody (CAB1085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using PDIA3 antibody (CAB1085) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PDIA3 antibody (CAB1085) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-ERp57 Antibody (CAB4376)	Human Protein Disulfide Isomerase A3 / PDIA3 ELISA Kit
PDIA3 Antibody (PACO01932)	Human PDIA3 (Protein Disulfide Isomerase A3) CLIA Kit (HUES00679)
PDIA3 Antibody (PACO11139)	Human PDIA3 (Protein Disulfide Isomerase A3) ELISA Kit (HUES02249)
	Human Protein disulfide-isomerase A3 (PDIA3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)