Anti-PHF10 Antibody (CAB15466)

Antibody Name:	Anti-PHF10 Antibody
Antibody SKU:	CAB15466
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PHF10 (NP_060758.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A431, LO2, Mouse pancreas, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PHF10 (NP_060758.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA AGPG AALS PRPC DSDP ATPG AQSP KDDN EDNS NDGT QPSK RRRM GSGD SSRS CETS SQDL GFSY YPAE NLIE YKWP PDET GEYY MLQE QVSE YLGV TSFK RKYP DLER RDLS HKEK LYLR ELNV ITET QCTL GLTA LRSD EVID LMIK EYPA KHAE YSVI LQEK ERQR ITDH YKEY SQMQ QQNT QKVE ASKV PEYI KKAA KKAA EFNS NLNR ERME ERRA YFDL QTHV IQVP QGKY KVLP TERT KVSS YPVA LIPG QFQE YYKR YSPD ELRY LPLN TALY EPPL DPEL PALD SDGD
Gene ID:	55274
Uniprot:	Q8WUB8
Cellular Location:	Nucleus
Calculated MW:	51kDa/55kDa/56kDa
Observed MW:	39kDa

Synonyms:

PHF10, BAF45A, XAP135

Background:

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.

UniProt Protein Function:	PHF10: Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Belongs to the SAYP family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 6q27 Cellular Component: nucleus Molecular Function:protein binding; zinc ion binding Biological Process: nervous system development; regulation of transcription, DNA-dependent; transcription, DNA-dependent
NCBI Summary:	This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WUB8
NCBI GenInfo Identifier:	194328734
NCBI Gene ID:	55274
NCBI Accession:	NP_060758.2
UniProt Secondary Accession:	Q8WUB8,Q2YDA3, Q53HG8, Q9BXD2, Q9NV26,
UniProt Related Accession:	Q8WUB8
Molecular Weight:	51,290 Da
NCBI Full Name:	PHD finger protein 10 isoform a
NCBI Synonym Full Names:	PHD finger protein 10
NCBI Official Symbol:	PHF10
NCBI Official Synonym Symbols:	BAF45A; XAP135
NCBI Protein Information:	PHD finger protein 10
UniProt Protein Name:	PHD finger protein 10
UniProt Synonym Protein Names:	BRG1-associated factor 45a; BAF45a; XAP135
Protein Family:	PHD finger protein
UniProt Gene Name:	PHF10
UniProt Entry Name:	PHF10_HUMAN

Western blot analysis of extracts of various cell lines, using PHF10 antibody (CAB15466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)