Phosphatidylcholine Colorimetric/Fluorometric Assay Kit (BN00805)

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SKU:
BN00805
  • Phosphatidylcholine Colorimetric/Fluorometric Assay Kit (BN00805)
  • Phosphatidylcholine Colorimetric/Fluorometric Assay Kit (BN00805)
€565

Description

ELISA Kit Technical ManualMSDS

Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. Assay Genie's Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the GenieRed probe to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). Assay Genie's Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).

Figure: Standard Curve, coloimetric and fluoroimetric

Key Information Description

Product SKU

BN00805

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

Assay Genie's Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample.

Features and Benefits

  • Simple procedure; takes less than ~40 minutes
  • Fast and convenient

Kit Components

  • PC Assay Buffer
  • GenieRed Probe
  • PC Hydrolysis Enzyme
  • PC Development Mix
  • PC Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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