Phosphatidylethanolamine Assay Kit (Fluorometric) (BN00730)

(No reviews yet) Write a Review
SKU:
BN00730
  • Phosphatidylethanolamine Assay Kit (Fluorometric) (BN00730)
  • Phosphatidylethanolamine Assay Kit (Fluorometric) (BN00730)
€787

Description

ELISA Kit Technical ManualMSDS

Phosphatidylethanolamine Assay Kit (Fluorometric)

Phosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues, and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low density lipoproteins in the liver. Assay Genie's Phosphatidylethanolamine assay kit is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (Ex/Em: 535/587). The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. Thus no other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. This assay kit can detect as low as 0.2 nmol per well.

Figure: (a) Phosphatidylethanolamine standard curve. (b) Phosphatidylethanolamine content in rat liver (100 µg wet tissue) and HEK 293 cells (25 µg protein). Sample preparation and assay was carried out according to kit protocol.

Key Information Description

Product SKU

BN00730

Size

100 assays

Kit Summary

  • Detection method- Fluorescence (Ex/Em 535/587 nm)
  • Sample type- cell and/or tissue lipid extracts
  • Species reactivity- Mammalian
  • Applications- This assay provides a convenient and non-ELISA alternative to detect Phosphatidylethanolamine amount in biological samples.

Detection Method

Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

This assay provides a convenient and non-ELISA alternative to detect Phosphatidylethanolamine amount in biological samples.

Features and Benefits

Simple, fast, does not require lenghty incubation times

Kit Components

  • PE Assay Buffer
  • PE Converter
  • PE Developer
  • PE Enzyme Mix
  • Probe
  • PE Standard (1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose