Anti-Phospho-PIK3R1/PIK3R3 (Tyr467/199) Antibody (PACO23894)

Antibody Name:	Phospho-PIK3R1/PIK3R3 (Tyr467/199) Antibody (PACO23894)
Antibody SKU:	PACO23894
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85/p55.

Form:	Liquid
Storage Buffer:	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot analysis of extracts from 293 cells untreated or treated with sorbitol using PI3 Kinase p85/p55 (phospho-Tyr467/199)Antibody.

Background:

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

Stoyanov, B. et al. (1995) Science 269, 690-3.
Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.

Synonyms:

p85, AGM7, p85-ALPHA, p55, p55-GAMMA

UniProt Protein Function:	PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Enzyme, regulatory subunit; Kinase, lipid Chromosomal Location of Human Ortholog: 5q13.1 Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; nucleus; cytosol; phosphoinositide 3-kinase complex Molecular Function:insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; insulin receptor substrate binding; neurotrophin TRKA receptor binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transcription factor binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein phosphatase binding; insulin receptor binding Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; viral reproduction; NFAT protein import into nucleus; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; positive regulation of tumor necrosis factor production; negative regulation of cell-matrix adhesion; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; induction of apoptosis via death domain receptors; B cell differentiation; T cell costimulation; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36
NCBI Summary:	Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
UniProt Code:	P27986
NCBI GenInfo Identifier:	118572681
NCBI Gene ID:	5295
NCBI Accession:	P27986.2
UniProt Secondary Accession:	P27986,Q15747, Q4VBZ7, Q53EM6, Q8IXA2, Q8N1C5, B3KT19 D3DWA0, E7EX19,
UniProt Related Accession:	P27986
Molecular Weight:	42,838 Da
NCBI Full Name:	Phosphatidylinositol 3-kinase regulatory subunit alpha
NCBI Synonym Full Names:	phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
NCBI Official Symbol:	PIK3R1
NCBI Official Synonym Symbols:	p85; AGM7; GRB1; IMD36; p85-ALPHA
NCBI Protein Information:	phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
UniProt Protein Name:	Phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Synonym Protein Names:	Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
UniProt Gene Name:	PIK3R1
UniProt Entry Name:	P85A_HUMAN

Antibodies	ELISA Kits
Phospho-PIK3R1/PIK3R3 (Y467/199) Antibody (PACO02973)	PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Colorimetric Cell-Based ELISA Kit
PIK3R1/PIK3R3 Antibody (PACO06748)	PI3-kinase p85-alpha/gamma Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)