Phosphoglucose Isomerase Activity Colorimetric Assay Kit (BN00988)

(No reviews yet) Write a Review
SKU:
BN00988
  • Phosphoglucose Isomerase Activity Colorimetric Assay Kit (BN00988)
  • Phosphoglucose Isomerase Activity Colorimetric Assay Kit (BN00988)
€601

Description

ELISA Kit Technical ManualMSDS

Phosphoglucose Isomerase Activity Colorimetric Assay Kit

Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy. In Assay Genie's Phosphoglucose Isomerase Activity Assay Kit, PGI converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.

Figure:(a). NADH Standard Curve (b).Phosphoglucose Isomerase activity in bovine liver, HeLa cell lysate & Positive Control. Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00988

Size

100 assays

Detection Method

Absorbance (450 nm)

Applications

The kit can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.

Features and Benefits

Sensitive assay for measuring phosphoglucose isomerase activity in various biological samples.

Kit Components

  • PGI Assay Buffer
  • PGI Substrate (Lyophilized)
  • PGI Enzyme Mix (Lyophilized)
  • PGI Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • PGI Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose