Plasma Kallikrein Inhibitor Screening Kit (Colorimetric) (BN01147)

(No reviews yet) Write a Review
SKU:
BN01147
€738

Description

ELISA Kit Technical ManualMSDS

Plasma Kallikrein Inhibitor Screening Kit (Colorimetric)

Plasma Prekallikrein (EC 3.4.21.34), is the glycosylated single chain zymogen precursor of the plasma serine protease Kallikrein (PK). It circulates with kininogen and is activated by Factor XIIa to Kallikrein in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma Prekallikrein is associated with diabetes and cardiovascular disease. Plasma Kallikrein inhibitors have been proposed as drugs to manage Hereditary Angioedema. Assay Genie's Plasma Kallikrein Inhibitor Screening Kit utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. In the presence of a Plasma Kallikrein inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Plasma Kallikrein.

Figure: (a) Progress curve of Plasma Kallikrein activity in the presence or absence of the PK Inhibitor. (b) IC50 of PK Inhibitor against Plasma Kallikrein activity was 21.7 ± 3 µU (n = 3). Assays were performed according to the kit protocol.

Key Info Discussion

Product SKU

BN01147

Size

100 assays

Detection Method

Colorimetric (OD 405 nm)

Species Reactivity

Mammalian

Applications

  • Screening/characterizing/studying Plasma Kallikrein inhibitors/activators
  • Simple and reliable test to screen Plasma Kallikdrein inhibitors/activators

Features & Benefits

  • High-throughput compatible
  • Includes Inhibitor Control

Kit Components

  • PK Assay Buffer
  • Human PK
  • PK Substrate
  • PK Inhibitor (1 mU/µl)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans.

View AllClose

Additional Information

Product type:
Assay
View AllClose

0 Reviews

View AllClose