Human VPS25 Recombinant Protein (RPPB5130)
- SKU:
- RPPB5130
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9BRG1
Description
| Product Name: | Human VPS25 Recombinant Protein |
| Product Code: | RPPB5130 |
| Size: | 20µg |
| Species: | Human |
| Target: | VPS25 |
| Synonyms: | Vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, Dermal papilla-derived protein 9, DERP9, EAP20, FAP20, MGC10540. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The VPS25 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF |
VPS25 contains the ESCRT-II complex (endosomal sorting complex required for transport II), that is needed for sorting of endosomal cargo proteins into MVBs and multivesicular body (MVB) formation. The MVB pathway facilitates transport of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex takes part in the recruitment of the ESCRT-III complex which is involved in transcription regulation, probably through its collaboration with ELL.
VPS25 Human Recombinant produced in E. coli is a single polypeptide chain containing 200 amino acids (1-176) and having a molecular mass of 23.3kDa.VPS25 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | VPS25: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. Belongs to the VPS25 family. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: nucleoplasm; mitochondrion; nucleolus; endosome membrane; nucleus; cytosol Molecular Function:protein binding; protein homodimerization activity; structural molecule activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport |
| NCBI Summary: | This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BRG1 |
| NCBI GenInfo Identifier: | 73920459 |
| NCBI Gene ID: | 84313 |
| NCBI Accession: | Q9BRG1.1 |
| UniProt Related Accession: | Q9BRG1 |
| Molecular Weight: | 20kDa |
| NCBI Full Name: | Vacuolar protein-sorting-associated protein 25 |
| NCBI Synonym Full Names: | vacuolar protein sorting 25 homolog |
| NCBI Official Symbol: | VPS25�� |
| NCBI Official Synonym Symbols: | DERP9; EAP20; FAP20�� |
| NCBI Protein Information: | vacuolar protein-sorting-associated protein 25 |
| UniProt Protein Name: | Vacuolar protein-sorting-associated protein 25 |
| UniProt Synonym Protein Names: | Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS25�� |
| UniProt Entry Name: | VPS25_HUMAN |
