HIV-1 nef Recombinant Protein (RPPB5562)
- SKU:
- RPPB5562
- Product Type:
- Recombinant Protein
- Species:
- Viral
- Uniprot:
- CAA41585
Description
| Product Name: | HIV-1 nef Recombinant Protein |
| Product Code: | RPPB5562 |
| Size: | 10µg |
| Species: | HIV-1 |
| Target: | nef |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content. |
| Formulation: | Lyophilized with 1% glycerol. |
| Solubility: | It is recommended to reconstitute the lyophilized HIV-1 nef in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized HIV-1 nef although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 nef should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by HPLC analysis and SDS-PAGE. |
| UniProt Code: | CAA41585 |
HIV-1 Nef is a 27kDa protein highly produced after a very short time after virus infection. HIV-1 Nef is an essential factor for efficient viral replication and phatogenesis.HIV-1 Nef facilitates virus replication and improves virions infectivity. HIV-1 Nef exerts pleiotropic effect. HIV-1 Nef down-modulates surface MHC-I molecules, decreases cell surface CD4 antigen by interacting with the Src family kinase LCK thereby inducing LCK-CD4 dissociation and by increasing clathrindependent endocytosis of this antigen to target it to lysosomial degradation. HIV-1 Nef guards the infected cell from apoptosis in order to keep it alive until the next virus generation is ready to strike. HIV-1 Nef protein bypasses host Tcell signaling by inducing a transcriptional program nearly identical to that of anti-CD3 cell activation.
The E.coli derived 27 kDa recombinant HIV-1 Nef Clade-B protein is a single non-glycosylated polypeptide chain.
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