Anti-PRDX3 Antibody (CAB13074)

Antibody Name:	Anti-PRDX3 Antibody
Antibody SKU:	CAB13074
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 63-256 of human PRDX3 (NP_006784.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, LO2, A-549, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 63-256 of human PRDX3 (NP_006784.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PAVT QHAP YFKG TAVV NGEF KDLS LDDF KGKY LVLF FYPL DFTF VCPT EIVA FSDK ANEF HDVN CEVV AVSV DSHF SHLA WINT PRKN GGLG HMNI ALLS DLTK QISR DYGV LLEG SGLA LRGL FIID PNGV IKHL SVND LPVG RSVE ETLR LVKA FQYV ETHG EVCP ANWT PDSP TIKP SPAA SKEY FQKV NQ
Gene ID:	10935
Uniprot:	P30048
Cellular Location:	Mitochondrion
Calculated MW:	25kDa/27kDa
Observed MW:	28kDa

Synonyms:

PRDX3, AOP-1, AOP1, HBC189, MER5, PRO1748, SP-22, prx-III

Background:

This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22.

UniProt Protein Function:	PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.11.1.15 Chromosomal Location of Human Ortholog: 10q25-q26 Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; early endosome; IkappaB kinase complex; cytosol Molecular Function:protein C-terminus binding; identical protein binding; protein binding; thioredoxin peroxidase activity; caspase inhibitor activity; alkyl hydroperoxide reductase activity; kinase binding; protein kinase binding Biological Process: negative regulation of kinase activity; mitochondrion organization and biogenesis; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; response to reactive oxygen species; peptidyl-cysteine oxidation; response to hydrogen peroxide; regulation of mitochondrial membrane potential; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; negative regulation of apoptosis; maternal placenta development
NCBI Summary:	This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]
UniProt Code:	P30048
NCBI GenInfo Identifier:	2507171
NCBI Gene ID:	10935
NCBI Accession:	P30048.3
UniProt Related Accession:	P30048
Molecular Weight:	26kDa
NCBI Full Name:	Thioredoxin-dependent peroxide reductase, mitochondrial
NCBI Synonym Full Names:	peroxiredoxin 3
NCBI Official Symbol:	PRDX3
NCBI Official Synonym Symbols:	AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
NCBI Protein Information:	thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Protein Name:	Thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Synonym Protein Names:	Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog
UniProt Gene Name:	PRDX3
UniProt Entry Name:	PRDX3_HUMAN

	Western blot analysis of extracts of various cell lines, using PRDX3 antibody (CAB13074) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of NIH-3T3 cells using PRDX3 Polyclonal Antibody (CAB13074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PRDX3 Polyclonal Antibody (CAB13074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PRDX3 Antibody (CAB3076)	Human PRDX3 / Peroxiredoxin 3 ELISA Kit
Anti-PRDX3 Antibody (CAB18022)[KO Validated]	Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit
Anti-PRDX3 Antibody (CAB18061)[KO Validated]
Anti-Peroxiredoxin 3 Antibody (CAB2398)
PRDX3 Antibody (PACO01369)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)