Acid Phosphatase Assay Kit – Information
Assay Genie’s non-radioactive, fluorimetric acid phosphatase assay (ACP) is based on the cleavage of methylumbelliferyl phosphate. Methylumbelliferone becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at 360/450 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)
Acid Phosphatase Assay Kit – Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.008 to 10 U/L for a 30 minute reaction.
- High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Acid Phosphatase Assay Kit – Data Sheet
|Kit Includes||Assay Buffer: 12 mL MUP Substrate: 120 µL Stop Reagent: 12 mL Standard: 120 µL|
|Kit Requires||Pipetting devices and accessories (e.g. multi-channel pipettor), black flat-bottom 96-well plates, centrifuge tubes and plate reader.|
|Method of Detection||FL360/450|
|Detection Limit||0.008 U/L|
|Samples||Biological (e.g. serum) samples|
|Protocol Length||30 min|
|Storage||Store all components at -20°C upon receiving|
|Shelf Life||6 months|
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.