ADP Assay Kit – Information
Assay Genie’s LumiDazzle ADP Assay Kit provides a rapid method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism.
ADP Assay Kit – Key Features
- Safe. Non-radioactive assay.
- Sensitive and accurate. As low as 0.02 μM ADP can be quantified.
- Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
ADP Assay Kit – Data Sheet
|Kit Includes||Assay Buffer: 10 mL Substrate: 120 mL Cosubstrate: 120 mL ATP Enzyme: 120 mL ADP Enzyme: 120 mL Standard: 100 mL 3 mM ADP|
|Kit Requires||Store all reagents at -20°C|
|Method of Detection||Luminescence|
|Detection Limit||0.02 μM|
|Protocol Length||20 min|
|Storage||Store all reagents at -20C|
|Shelf Life||12 months|