Ammonia Assay Kit – Information
Assay Genie’s ammonia assay is designed to directly measure NH3 and NH4+. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at Ex/Em = 360/450 nm is directly proportionate to the NH3 concentration in the sample.
For quantitative determination of ammonia/ammonium concentration.
Ammonia Assay Kit – Key Features
- High sensitivity and wide linear range. Use 20 μL sample. Linear detection range 24 to 1000 μM ammonia.
- Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of ammonia within 30 minutes.
Ammonia Assay Kit – Data Sheet
|Kit Includes||Assay Buffer: 20 mL Enzyme: 120 µL Ketoglutarate: 120 µL Standard: 400 µL. NADH Reagent: Dried|
|Kit Requires||Pipeting devices, and clear flat-bottom 96-well plates and optical density plate reader for colorimetric assays; black flatbottom 96-well plate and fluorescence intensity plate reader for fluorimetric assays|
|Method of Detection||OD340nm, or FL360/450nm|
|Detection Limit||24 μM|
|Samples||Serum, plasma, urine, saliva, cell culture etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C|
|Shelf Life||6 months|
Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Simple, direct and automation-ready procedures for measuring NH3 are popular in research and drug discovery.