D-Lactate Assay Kit – Information
Assay Genie’s Colorimetric D-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
D-Lactate Assay Kit – Key Features
- Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
D-Lactate Assay Kit – Data Sheet
|Kit Includes||Assay Buffer: 10 mL NAD Solution: 1 mL Enzyme A: 120 mL MTT Solution: 1.5 mL Enzyme B: 120 mL Standard: 1.0 mL 20 mM D-lactate|
|Kit Requires||Pipeting (multi-channel) devices. Clear-bottom 96-well plates and plate reader.|
|Method of Detection||OD565 nm|
|Detection Limit||0.05 mM|
|Samples||Serum, plasma, cell culture media etc|
|Protocol Length||20 min|
|Storage||Store all reagents at -20°C|
|Shelf Life||6 months|
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.