Ethanol Assay Kit – Information
Ethanol assay kit (colorimetric) is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample
For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism.
Ethanol Assay Kit – Key Features
- Sensitive and accurate. Detection limit 0.0008 vol % (140 μM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay.
- Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Ethanol Assay Kit – Data Sheet
|Kit Includes||Assay Buffer: 10 mL Enzyme A: Lyophilized NAD Solution: 1 mL Enzyme B: 120 mL MTT Solution: 1.5 mL Enzyme Buffer: 150 mL Standard: 1.5 mL 1% Ethanol Stop Reagent: 12 mL|
|Kit Requires||Pipeting (multi-channel) devices. Clear flat-bottom 96-well plates and plate reader.|
|Method of Detection||OD565nm (Enzymatic)|
|Samples||Serum, plasma, urine, saliva samples etc|
|Protocol Length||30 min|
|Storage||Store all reagents at -20°C|
|Shelf Life||6 months|
Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable.