Assay Genie’s formate assay kit is based on formate dehydrogenase catalyzed oxidation of formate, which generates carbon dioxide and NADH that reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to formate concentration in the sample.
Direct determination of formate concentrations in biological (e.g. urine, blood, etc.) and other samples.
- Fast and sensitive. Use of 10 µL sample. Linear detection range 0.050 to 5 mM Formate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
|Kit Includes||Assay Buffer: 10 mL NAD/MTT: 1 mL Enzyme A: 120 µL Standard: 0.5 mL 20 mM Formate Enzyme B: 120 µL|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates (e.g. VWR cat# 82050-760), and plate reader.|
|Method of Detection||OD565nm|
|Detection Limit||50 μM|
|Samples||Urine, Serum etc|
|Protocol Length||Approximately 60 min|
|Storage||Store all components at -20°C upon receiving|
|Shelf Life||6 months|
FORMATE (HCOO–) is the anion derived from formic acid, the simplest carboxylic acid. It is also the metabolic byproduct of formaldehyde metabolism in our body, and the eventual metabolic byproduct of methanol which is first broken down to formaldehyde. At high levels, formate is neurotoxic to the central nervous system and can cause blindness, coma, and death. Although naturally present in the body at low levels, elevated levels of formate may be used as an indicator of formaldehyde exposure and methanol poisoning.