Glucose-6-Phosphate Assay Kit – Information
Assay Genie’s Glucose-6-Phosphate Assay Kit provides a simple, and automation-ready procedure for measuring G6P concentration. G6P is oxidized by glucose-6-phosphate dehydrogenase and the formed NADPH is coupled to the formazan (WST-8) chromogen. The intensity of the product color, measured at 460 nm, is proportional to the G6P concentration in the sample.
For quantitative determination of glucose-6-phosphate in biological samples.
Glucose-6-Phosphate Assay Kit – Key Features
- Fast and sensitive. Linear detection range: 10 to 1000 μM G6P.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Glucose-6-Phosphate Assay Kit – Data Sheet
|Kit Includes||Assay Buffer: 10 mL Enzyme A: 120 µL NADP/WST8: 1 mL Enzyme B: 120 µL Standard (100 mM G6P): 100 µL|
|Kit Requires||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well centrifuge tubes and plate reader|
|Method of Detection||OD460nm|
|Detection Limit||10 uM|
|Samples||Plasma, serum, tissue and culture media etc|
|Protocol Length||20 min|
|Storage||Store all kit components at -20 °C.|
|Shelf Life||6 months|
Glucose-6-Phosphate (g6p) is glucose sugar phosphorylated on carbon 6. Most of the glucose entering cells is phosphorylated to G6P. G6P has three primary fates within the cell. It lies at the start of two major metabolic pathways: glycolysis and the pentose phosphate pathway. In addition to these metabolic pathways, glucose 6-phosphate may also be converted to glycogen or starch for storage.