Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric) (BN01046)

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  • Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric) (BN01046)
  • Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric) (BN01046)
  • Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric) (BN01046)


ELISA Kit Technical ManualMSDS

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)

Protein tyrosine phosphatases (PTPase(s), EC are a diverse family of enzymes that catalyze the removal of a phosphate moiety from protein phosphotyrosine residues. Phosphorylation and dephosphorylation of tyrosine residues is a common post-translational modification that serves as a regulatory switch for many enzymes, receptors and signal transduction pathways. PTPs can be classified as intracellular (non-receptor) or receptor-like based upon their cellular localization, however all classical PTPs share a common catalytic mechanism utilizing a nucleophilic cysteine residue. As key regulators of cellular signaling cascades, PTPs affect cell growth, differentiation, migration and metabolism. Dysfunction of certain PTPs has been linked to several human diseases. For example, the intracellular tyrosine phosphatase PTP1B is a negative regulator of the insulin signaling cascade and has also been demonstrated to be dramatically overexpressed in human breast, colon and ovarian cancers. Assay Genie's PTPase Activity Assay Kit enables rapid measurement of PTPase activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). A broad-spectrum PTPase inhibitor is provided for verification of specific activity in complex biological matrices, where serine/threonine phosphatases may contribute to substrate metabolism. Unlike ELISAs or malachite green-based approaches, the assay is homogeneous, continuous and does not require complicated sample processing or desalting to eliminate free phosphate. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 0.1 mU PTPase activity

Figure: (a) Fluorescence Standard curve. One mole of Fluorescence Standard corresponds to the metabolism of one mole of PTPase Substrate with release of one mole free inorganic phosphate. (b) Reaction kinetics of PTPase Substrate metabolism in homogenates of human placenta (5 μg) and rat prefrontal cortex (10 μg) in the presence and absence of the PTPase inhibitor suramin. (c) Quantification of PTPase activity in samples (mean ± SEM of 4 or more independent replicates). Assays were performed according to the kit protocol.

Key Information Description

Product SKU



100 assays

Kit Summary

  • Detection method- Fluorescence (368/440)
  • Species reactivity- Mammalian
  • Applications- The assay is designed to measure PTPase activity in biological samples with detection sensitivity ~100 µU

Detection Method

Fluorescence (368/460 nm)

Species Reactivity



Rapid assessment of PTPase activity in biological samples or recombinant PTPase preparations

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • The kit is more sensitive than colorimetric assays using phosphate detection.

Kit Components

  • PTPase Assay Buffer
  • Disulfide Reducing Agent (DTT)
  • Fluorescent Standard
  • PTPase Inhibitor (Suramin)
  • PTPase Substrate
  • PTPase Positive Control

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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