Proteinase K

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SKU:
CRPB02503
$55

Description

 

Proteinase K

Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction . Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.

Information Description

Product Name

Proteinase K

Product Description

No DNase contamination detected by digestion of λ DNA at 37 ℃ for 6 hours. No RNases contamination detected by digestion of λ DNA at 25℃ for 16 hours. Host genomic DNA/RNA is no residual detected by PCR

SKU Number

CRPB02503

Size

20 mg, 1 g

Bio-Activity

Measured by its digestion activity. The activity is >32U/mg. One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 µmol of L-tyrosine.

Purity

> 95% by SDS-PAGE.

Endotoxin

Please contact us for more information.

Formulation

Lyophilized powder is recommended to dissolve in in 50mM Tris-Hcl, 30mM NaCl, 50% Glycerol, pH 7.2-7.4.

Species

Tritirachium album

Expressed Host

Pichia pastoris

Tag

Tag Free

Storage Conditions

4℃ for up to 6 months

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