Anti-PSMB9 Antibody (CAB1771)

Antibody Name:	Anti-PSMB9 Antibody
Antibody SKU:	CAB1771
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human PSMB9 (NP_002791.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	THP-1, DU145, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human PSMB9 (NP_002791.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLRA GAPT GDLP RAGE VHTG TTIM AVEF DGGV VMGS DSRV SAGE AVVN RVFD KLSP LHER IYCA LSGS AADA QAVA DMAA YQLE LHGI ELEE PPLV LAAA NVVR NISY KYRE DLSA HLMV AGWD QREG GQVY GTLG GMLT RQPF AIGG SGST FIYG YVDA AYKP GMSP EECR RFTT DAIA LAMS RDGS SGGV IYLV TITA AGVD HRVI LGNE LPKF YDE
Gene ID:	5698
Uniprot:	P28065
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	22kDa/23kDa
Observed MW:	22kDa

Synonyms:

PSMB9, LMP2, PSMB6i, RING12, beta1i

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.

UniProt Protein Function:	PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.25.1; Nuclear receptor co-regulator; Protease; Proteasome complex Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]
UniProt Code:	P28065
NCBI GenInfo Identifier:	417529
NCBI Gene ID:	5698
NCBI Accession:	P28065.2
UniProt Secondary Accession:	P28065,P28076,
UniProt Related Accession:	P28065
Molecular Weight:
NCBI Full Name:	Proteasome subunit beta type-9
NCBI Synonym Full Names:	proteasome 20S subunit beta 9
NCBI Official Symbol:	PSMB9
NCBI Official Synonym Symbols:	LMP2; PRAAS3; PSMB6i; RING12; beta1i
NCBI Protein Information:	proteasome subunit beta type-9
UniProt Protein Name:	Proteasome subunit beta type-9
UniProt Synonym Protein Names:	Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Proteasome chain 7; Proteasome subunit beta-1i; Really interesting new gene 12 protein
Protein Family:	Latent membrane protein
UniProt Gene Name:	PSMB9
UniProt Entry Name:	PSB9_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMB9 antibody (CAB1771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunofluorescence analysis of MCF-7 cells using PSMB9 antibody (CAB1771).

Antibodies	ELISA Kits
Anti-PSB9 / LMP2 Antibody (CAB9549)	Human PSMB9 / Proteasome subunit beta type-9 ELISA Kit
PSMB9 Antibody (PACO11539)	Human Proteasome subunit beta type-9 (PSMB9) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)