Anti-PUF60 Antibody (CAB6709)

Antibody Name:	Anti-PUF60 Antibody
Antibody SKU:	CAB6709
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, A-549, Mouse brain, Mouse thymus, Mouse spleen, Rat lung, Rat liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DPTT GKHK GYGF IEYE KAQS SQDA VSSM NLFD LGGQ YLRV GKAV TPPM PLLT PATP GGLP PAAA VAAA AATA KITA QEAV AGAA VLGT LGTP GLVS PALT LAQP LGTL PQAV MAAQ APGV ITGV TPAR PPIP VTIP SVGV VNPI LASP PTLG LLEP KKEK EEEE LFPE SERP EMLS EQEH MSIS GSSA RHMV MQKL LRKQ ESTV MVLR NMVD PKDI DDDL EGEV TEEC GKFG AVNR VIIY QEKQ GEEE DAEI IVKI FVEF SIAS ETHK AIQA LNGR WFAG RKVV AEVY DQER FDNS DLSA
Gene ID:	22827
Uniprot:	Q9UHX1
Cellular Location:	Nucleus
Calculated MW:	54kDa/55kDa/57kDa/58kDa/59kDa
Observed MW:	65kDa

Synonyms:

PUF60, FIR, RoBPI, SIAHBP1, VRJS

Background:

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex Molecular Function:identical protein binding; protein binding; DNA binding; nucleotide binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing Disease: Verheij Syndrome
NCBI Summary:	This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
UniProt Code:	Q9UHX1
NCBI GenInfo Identifier:	74761960
NCBI Gene ID:	22827
NCBI Accession:	Q9UHX1.1
UniProt Secondary Accession:	Q9UHX1,Q969E7, Q96D94, Q96H63, Q99628, Q9NZA0, Q9UJY7 A8K8K8,
UniProt Related Accession:	Q9UHX1
Molecular Weight:	559
NCBI Full Name:	Poly(U)-binding-splicing factor PUF60
NCBI Synonym Full Names:	poly-U binding splicing factor 60KDa
NCBI Official Symbol:	PUF60
NCBI Official Synonym Symbols:	FIR; VRJS; RoBPI; SIAHBP1
NCBI Protein Information:	poly(U)-binding-splicing factor PUF60; poly(U)-binding-splicing factor PUF60; Siah binding protein 1; FBP interacting repressor; Ro ribonucleoprotein-binding protein 1; pyrimidine tract binding splicing factor; FUSE-binding protein-interacting repressor
UniProt Protein Name:	Poly(U)-binding-splicing factor PUF60
UniProt Synonym Protein Names:	60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1
Protein Family:	Poly(U)-binding-splicing factor
UniProt Gene Name:	PUF60
UniProt Entry Name:	PUF60_HUMAN

	Western blot analysis of extracts of various cell lines, using PUF60 antibody (CAB6709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (CAB6709) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using PUF60 antibody (CAB6709). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug PUF60 antibody (CAB6709). Western blot was performed from the immunoprecipitate using PUF60 antibody (CAB6709) at a dilition of 1:500.

Antibodies

PUF60 Antibody (PACO11609)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)