Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit (BN00929)

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SKU:
BN00929
  • Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit (BN00929)
  • Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit (BN00929)
€696

Description

ELISA Kit Technical ManualMSDS

Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. Assay Genie provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.

Figure: (a) Pyruvate Standard Curve obtained using kit protocol (b) PK sample kinetics

Key Information Description

Product SKU

BN00929

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The kit detects 0.1 mU/ml pyruvate kinase.

Features and benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • PK Assay Buffer
  • GenieRedTM Probe (in DMSO)
  • PK Enzyme Mix
  • PK Substrate Mix
  • PK Positive Control (~18 mU)
  • Pyruvate Standard (100 nmol/µl)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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