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Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal (CABE009)

SKU:
CABE009
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
  • Protein Tags Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal CABE009
  • Protein Tags Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal CABE009
  • Protein Tags Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal CABE009
€349
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Description

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Antibody Name:Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal
Antibody SKU:CABE009
Antibody Size:100 uL
Application:WB IF IP
Reactivity:
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to Myc tag.
Application:WB IF IP
Recommended Dilution:WB 1:1000 - 1:10000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:
Positive Samples:293T
Immunogen:A synthetic peptide corresponding to Myc tag.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EQKL ISEE DL
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:Refer to Figures
Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal (CABE009)Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Myc-Tag antibody (CABE009) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal (CABE009)Immunofluorescence analysis of HeLa-Myc-CandHeLa cells using Rabbit anti Myc-Tag pAb-C-terminal (CABE009) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal (CABE009)Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Rabbit anti Myc-Tag pAb-C-terminal antibody (CABE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb-C-terminal antibody (CABE009) at a dilition of 1:50000.
Synonyms:Myc, Myc tag, Myc-tag
Background:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

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