Rat Neurokinin-A PharmaGenie ELISA Kit (SBRS0140)
- SKU:
- SBRS0140
- Product Type:
- ELISA Kit
- Reactivity:
- Rat
- Applications:
- ELISA
- ELISA Type:
- Competitive
Description
Rat Neurokinin-A PharmaGenie ELISA - Kit Information
Neurokinin A, aka substance K, is a peptide hormone belonging to the tachykinin family, which also includes Substance P and Neurokinin B. All the peptides from tachykinin families are derived from two preprotachykinin genes - the PPT-A gene and PPT-B gene. The former encodes the sequences of Substance P. Neurokinin A. and Neuropeptide K and the latter encodes the sequence of Neurokinin B. Neuropeptide K and Neuropeptide gamma are N-terminally longer versions of neurokinin A. Neurokinin A has played a variety of roles in human. For example, it is reported that Neurokinin A may mediate bronchoconstriction through the non-adrenergic non-cholinergic nervous system in rat. There has been no clinical application reported for neurokinin A.
Rat Neurokinin-A PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS0140 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC. |
| Uniprot: | P06767 |
| Gene ID: | 24806 |
| Gene Names: | NKA / NKNA / TAC1 / TAC2 |
| Synonyms: | Protachykinin-1 (PPT) [Cleaved into: Substance P Neurokinin A (NKA) (Neuromedin L) (Substance K) Neuropeptide K (NPK) Neuropeptide gamma C-terminal-flanking peptide] |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 pg/ml |
| Sensitivity: | 0.8 pg/ml |
| Recommended Dilution: | Human: 2X, Mouse: 2X, Rat: 2X |
| Storage/Stability: | Standard, Biotinylated Neurokinin A peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Standard Peptide |
| 4. | Assay Diluent(s) |
| 5. | Biotinylated Peptide |
| 6. | HRP-Streptavidin |
| 7. | TMB One-Step Substrate |
| 8. | Stop Solution |
| 9. | Assay Diagram |
| 10. | Positive Control Sample |
| 11. | Capture Antibody |
| 12. | Technical Manual |
Other materials and equipment required:
The Assay Genie Rat Neurokinin-A ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
