Rat Renin Activity Fluorometric Assay Kit (BN01022)

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SKU:
BN01022
  • Rat Renin Activity Fluorometric Assay Kit (BN01022)
  • Rat Renin Activity Fluorometric Assay Kit (BN01022)
  • Rat Renin Activity Fluorometric Assay Kit (BN01022)
€738

Description

ELISA Kit Technical ManualMSDS

Rat Renin Activity Fluorometric Assay Kit

Renin (EC 3.4.23.15), also known as angiotensinogenase, is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. In addition to the systemic RAS, locally expressed RAS has been found in the kidney, heart and nervous system. An overactive renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension. In Assay Genie's Rat Renin Activity Assay Kit, Rat Renin and other proteases hydrolyze a FRET substrate resulting in fluorescence at Ex/Em = 328/552 nm. In the presence of a Rat Renin-Specific Inhibitor, hydrolysis of the substrate is due to only the activity of other proteases. The difference between the total activity and the activity in the presence of Rat Renin-Specific Inhibitor gives the Rat Renin Activity in the sample. This rapid, simple & sensitive kit can detect renin activity as low as 0.12 mU recombinant rat renin.

Figure: (a) EDANS Standard Curve, (b) Renin Activity in Positive Control & (c) Renin Activity in Rat Kidney (RK) (28 μg) lysate and Rat Brain (RB) (9.2 μg) lysate with (+) and without (-) Inhibitor. Assays were performed following this kit protocol.

Key Information Description

Product SKU

BN01022

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 328/552 nm)

Applications

Detects Rat Renin Activity

Features and Benefits

  • Rapid, simple & convenient
  • The assay can detect rat renin activity as low as 0.12 mU recombinant rat renin.

Kit Components

  • Renin Assay Buffer
  • Homogenization Buffer
  • Rat Renin Substrate
  • Rat Renin (Lyophilized)
  • Rat Renin Inhibitor (Lyophilized)
  • EDANS Standard (100 µM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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