Guanine-N7_meth Protein (His Tag) SARS-Cov-2 (RPES0009)

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SKU:
RPES0009
€300

Description

Background

The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.

Information Description
Product Name:
SARS-Cov-2 Guanine-N7_meth Protein (His Tag)
Size:

10µg

SKU:

RPES0009

Activity:
Synonym:
SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
Protein Construction:
SARS-Cov-2 Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.
Sequence:
Ala1-Gln527
Fusion tag:
N-6His
Accession:
YP_009725309.1
Species:

Virus

Expressed Host:
E.coli
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Endotoxin:
< 1.0 EU per µg as determined by the LAL method.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Mol Mass:
62.9 kDa
AP Mol Mass:
60 kDa
Formulation:
Supplied as a 0.2 uM filtered solution of PBS, 10% Glycerol, pH 7.4.
Reconstitution:
Please refer to the printed manual for detailed information.
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