Recombinant SARS-CoV-2 Nucleoprotein Protein (His-Tag) (RPES0048)

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SKU:
RPES0048
€1,479

Description

Information Description
Background:
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Size:

100µg

Product Name:
SARS-Cov-2 Nucleoprotein / NP Protein (His Tag)
Activity:
Synonym:
2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Protein Construction:
A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag.
Sequence:
Met1-Ala422
Fusion tag:
C-6His
Accession:
NP_828858.1
Species:

Virus

Expressed Host:
Baculovirus-Insect Cells
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Purity:
> 80 % as determined by reducing SDS-PAGE.
Endotoxin:
< 1.0 EU per µg as determined by the LAL method.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months.
Mol Mass:
47.5 kDa
AP Mol Mass:
47.1 kDa
Formulation:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Reconstitution:
Please refer to the printed manual for detailed information.
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