Recombinant SARS-CoV-2 Spike S1 Protein (Fc Tag) (RPES0044)

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SKU:
RPES0044
€982

Description

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Background:
Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Size:

100µg

Product Name:
SARS-Cov-2 S1 Protein (Fc Tag)
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2ug/ml (100uL/well) can bind SARS-Cov-2 S1 Protein (Fc Tag) (RPES0044), the EC50 of SARS-Cov-2 S1 Protein (Fc Tag) is 25-60 ug/ml.
Synonym:
S1 protein; 2019-nCoV S1 protein; coronavirus S1 Protein; cov S1 Protein
Protein Construction:
A DNA sequence encoding the 2019-nCoV spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with the Fc region of human IgG1 at the C-terminus.
Sequence:
Val16-Arg685
Fusion tag:
C-Fc
Accession:
YP_009724390.1
Species:

Virus

Expressed Host:
HEK293 Cells
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Endotoxin:
< 1.0 EU per µg as determined by the LAL method.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months.
Mol Mass:
101.7 kDa
AP Mol Mass:
Formulation:
Lyophilized from sterile PBS, pH 7.4.
Reconstitution:
Please refer to the printed manual for detailed information.
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