Recombinant Human Annexin A1/ANXA1 Protein (RPES2602)

SKU:
RPES2602
€196

Description

ELISA Kit Technical Manual

Recombinant Human Annexin A1/ANXA1 Protein

Background:Annexin A1 is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis. Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity. Annexin A1 also has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development. ANXA1 is strongly expressed on the cell membrane and occasionally in the cytoplasm of tumor cells in 97% of samples from patients with hairy cell leukemia.
Name:Recombinant Human Annexin A1/ANXA1 Protein
Size:10µg
Assay Genie SKU:RPES2602
Synonyms:Annexin A1; Annexin I; Annexin; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 Inhibitory Protein; p35
Species:Human
Expression host:E.coli
Sequence:Ala2-Asn346
Accession:P04083
Mol Mass:38.6 kDa
AP Mol Mass:34 kDa
Tag:
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
UniProt Protein Details:

Protein type:Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: apical plasma membrane; basolateral plasma membrane; cell surface; cornified envelope; cytoplasm; cytoplasmic vesicle membrane; early endosome membrane; endosome; extracellular region; extracellular space; extrinsic to external side of plasma membrane; extrinsic to membrane; focal adhesion; lateral plasma membrane; mast cell granule; mitochondrial membrane; nucleoplasm; nucleus; phagocytic cup; plasma membrane; protein complex; sarcolemma; vesicle

Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; double-stranded DNA-dependent ATPase activity; helicase activity; phospholipase A2 inhibitor activity; phospholipid binding; protein binding; protein binding, bridging; protein homodimerization activity; receptor binding; single-stranded DNA binding; single-stranded RNA binding; structural molecule activity

Biological Process: actin cytoskeleton reorganization; adaptive immune response; alpha-beta T cell differentiation; arachidonic acid secretion; cell surface receptor linked signal transduction; DNA duplex unwinding; DNA strand renaturation; endocrine pancreas development; G-protein signaling, coupled to cyclic nucleotide second messenger; gliogenesis; inflammatory response; innate immune response; insulin secretion; keratinocyte differentiation; monocyte chemotaxis; muscle contraction; myoblast migration involved in skeletal muscle regeneration; negative regulation of apoptosis; negative regulation of exocytosis; negative regulation of T-helper 2 cell differentiation; neutrophil homeostasis; peptide cross-linking; phagocytosis; positive regulation of interleukin-2 production; positive regulation of neutrophil apoptosis; positive regulation of prostaglandin biosynthetic process; positive regulation of T cell proliferation; positive regulation of T-helper 1 cell differentiation; positive regulation of vesicle fusion; prostate gland development; regulation of cell shape; regulation of hormone secretion; regulation of inflammatory response; regulation of interleukin production; regulation of leukocyte migration; response to drug; response to estradiol stimulus; response to peptide hormone stimulus; response to X-ray; signal transduction

NCBI Summary:This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
UniProt Code:P04083
NCBI GenInfo Identifier:113944
NCBI Gene ID:301
NCBI Accession:P04083.2
UniProt Secondary Accession:P04083,P10107,
UniProt Related Accession:P04083
Molecular Weight:38,714 Da
NCBI Full Name:Annexin A1
NCBI Synonym Full Names:annexin A1
NCBI Official Symbol:ANXA1  
NCBI Official Synonym Symbols:ANX1; LPC1  
NCBI Protein Information:annexin A1
UniProt Protein Name:Annexin A1
UniProt Synonym Protein Names:Annexin I; Annexin; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I
Protein Family:Annexin
UniProt Gene Name:ANXA1  
UniProt Entry Name:ANXA1_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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