Carbonic Anhydrase 3 (CA3) belongs to the Alpha-Carbonic Anhydrase family that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and it is present at high levels in skeletal muscle with much lower levels found in cardiac and smooth muscle. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. CA3 is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
Carbonic Anhydrase 3/CA3 Recombinant Protein (RPES4229)
Carbonic Anhydrase 3, Carbonate Dehydratase III, Carbonic Anhydrase III, CA-III, CA3,Car3
<1.0 EU per µg as determined by the LAL method.
Recombinant Human Carbonic Anhydrase 3 is produced by our E.coli expression system and the target gene encoding Ala2-Lys260 is expressed with a 6His tag at the C-terminus.
> 95 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Supplied as a 0.2 µm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5.
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.