Recombinant Human Myeloperoxidase/MPO Protein (His Tag) (RPES5028)

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RPES5028
€196

Description

ELISA Kit Technical Manual

Recombinant Human Myeloperoxidase/MPO Protein (His Tag)

Background:Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational processing of MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of the signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen-containing phagosomes.
Name:Recombinant Human Myeloperoxidase/MPO Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES5028
Synonyms:Myeloperoxidase; MPO
Species:Human
Expression host:Human Cells
Sequence:Ala49-Ser745
Accession:P05164
Mol Mass:80.3 kDa
AP Mol Mass:85-95 kDa
Tag:C0His
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM Tris, 150mM NaCl, pH8.0 .
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function:heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; defense response; response to yeast; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease

NCBI Summary:Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
UniProt Code:P05164
NCBI GenInfo Identifier:129825
NCBI Gene ID:4353
NCBI Accession:P05164.1
UniProt Secondary Accession:P05164,Q14862, Q4PJH5, Q9UCL7, A1L4B8,
UniProt Related Accession:P05164
Molecular Weight:87,248 Da
NCBI Full Name:Myeloperoxidase
NCBI Synonym Full Names:myeloperoxidase
NCBI Official Symbol:MPO  
NCBI Protein Information:myeloperoxidase
UniProt Protein Name:Myeloperoxidase
Protein Family:Myeloperoxidase
UniProt Gene Name:MPO  
UniProt Entry Name:PERM_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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