Recombinant Human Novel Coronavirus Nucleoprotein(N)(T205I)(Active)(RPCO00001)
- SKU:
- RPCO00001
- Product Type:
- Recombinant Protein
- Target:
- N
- Synonyms:
- N
- Synonyms:
- Nucleocapsid protein
- Synonyms:
- NC
- Synonyms:
- Protein N
- Species:
- SARS-CoV-2
- Source:
- E.coli
- Uniprot:
- P0DTC9
Frequently bought together:
Description
Product Name: | Recombinant Human Novel Coronavirus Nucleoprotein(N)(T205I) (Active) |
Product Code: | RPCO00001 |
Product Size: | 100µg |
Target Name: | N |
Synonyms: | N, Nucleocapsid protein, NC, Protein N |
Species: | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Source: | E.coli |
Uniprot: | P0DTC9 |
Activity: | 1.) Measured by its binding ability in a functional ELISA. Immobilized N (RPCO00001) at 2 µg/ml can bind N Mouse Monoclonal Antibody, the EC50 of N Mouse Monoclonal Antibody is 5.660-6.932 ng/ml. 2.) Measured by its binding ability in a functional ELISA. Immobilized N (RPCO00001) at 2 µg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody is 29.34-40.67 ng/ml. |
Expression Region: | 1-419aa (T205I) |
Protein Length: | Full Length |
Target Sequence: | MSDN GPQN QRNA PRIT FGGP SDST GSNQ NGER SGAR SKQR RPQG LPNN TASW FTAL TQHG KEDL KFPR GQGV PINT NSSP DDQI GYYR RATR RIRG GDGK MKDL SPRW YFYY LGTG PEAG LPYG ANKD GIIW VATE GALN TPKD HIGT RNPA NNAA IVLQ LPQG TTLP KGFY AEGS RGGS QASS RSSS RSRN SSRN STPG SSRG ISPA RMAG NGGD AALA LLLL DRLN QLES KMSG KGQQ QQGQ TVTK KSAA EASK KPRQ KRTA TKAY NVTQ AFGR RGPE QTQG NFGD QELI RQGT DYKH WPQI AQFA PSAS AFFG MSRI GMEV TPSG TWLT YTGA IKLD DKDP NFKD QVIL LNKH IDAY KTFP PTEP KKDK KKKA DETQ ALPQ RQKK QQTV TLLP AADL DDFS KQLQ QSMS SADS TQA |
Tag Information: | N-terminal 6xHis-tagged |
Mol. Weight: | 49.7 kDa |
Research Area: | Microbiology |
Purity: | Greater than 93% as determined by SDS-PAGE. |
Form: | Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
Buffer: | Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Reconstitution: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage Conditions: | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Shelf Life: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Notes: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |